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Cold Spring Harb Protoc. 2014 Mar 1;2014(3):304-6. doi: 10.1101/pdb.prot073437.

Producing and concentrating lenti-Cre for mouse infections.

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1
Molecular Pathology Unit and Center for Cancer Research, Massachusetts General Hospital, Department of Pathology, Harvard Medical School, Charlestown, Massachusetts 02129;

Abstract

Lentiviral vectors offer versatility as vehicles for gene delivery. They can transduce a wide range of cell types and integrate into the host genome, which results in long-term expression of the transgene (Cre) both in vitro and in vivo. This protocol describes how lentiviral particles are produced, purified, and concentrated.

PMID:
24591688
PMCID:
PMC4169239
DOI:
10.1101/pdb.prot073437
[Indexed for MEDLINE]
Free PMC Article
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