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EMBO Rep. 2014 Apr;15(4):428-37. doi: 10.1002/embr.201338128. Epub 2014 Mar 3.

FIP5 phosphorylation during mitosis regulates apical trafficking and lumenogenesis.

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  • 1Department of Cell and Developmental Biology, School of Medicine, Anschutz Medical Campus University of Colorado Denver, Aurora, CO, USA.

Abstract

Apical lumen formation is a key step during epithelial morphogenesis. The establishment of the apical lumen is a complex process that involves coordinated changes in plasma membrane composition, endocytic transport, and cytoskeleton organization. These changes are accomplished, at least in part, by the targeting and fusion of Rab11/FIP5-containing apical endosomes with the apical membrane initiation site (AMIS). Although AMIS formation and polarized transport of Rab11/FIP5-containing endosomes are crucial for the formation of a single apical lumen, the spatiotemporal regulation of this process remains poorly understood. Here, we demonstrate that the formation of the midbody during cytokinesis is a symmetry-breaking event that establishes the location of the AMIS. The interaction of FIP5 with SNX18, which is required for the formation of apical endocytic carriers, is inhibited by GSK-3 phosphorylation at FIP5-T276. Importantly, we show that FIP5-T276 phosphorylation occurs specifically during metaphase and anaphase, to ensure the fidelity and timing of FIP5-endosome targeting to the AMIS during apical lumen formation.

PMID:
24591568
PMCID:
PMC3989674
DOI:
10.1002/embr.201338128
[PubMed - indexed for MEDLINE]
Free PMC Article
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