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Methods Mol Biol. 2014;1140:211-37. doi: 10.1007/978-1-4939-0354-2_17.

Data collection for crystallographic structure determination.

Author information

1
NE-CAT and Department of Chemistry and Chemical Biology, Cornell University, Argonne National Laboratory, Argonne, IL, 60439, USA.

Abstract

Diffraction data measurement is the final experimental step of crystal structure analysis; all subsequent stages are computational. Good-quality data, optimized for a particular application, make the structure solution and refinement easier and enhance the accuracy of the final models. This chapter describes the principles of the rotation method of data collection and discusses various scenarios that are useful for different types of applications, such as anomalous phasing, molecular replacement, ligand identification, etc. Some typical problems encountered in practice are also discussed.

PMID:
24590721
DOI:
10.1007/978-1-4939-0354-2_17
[Indexed for MEDLINE]

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