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Nucleic Acids Res. 2014 Apr;42(8):e73. doi: 10.1093/nar/gku169. Epub 2014 Mar 3.

MyTaxa: an advanced taxonomic classifier for genomic and metagenomic sequences.

Author information

1
Centre for Bioinformatics and Computational Genomics, and School of Biology, Georgia Institute of Technology, Atlanta, GA 30332, USA and School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.

Abstract

Determining the taxonomic affiliation of sequences assembled from metagenomes remains a major bottleneck that affects research across the fields of environmental, clinical and evolutionary microbiology. Here, we introduce MyTaxa, a homology-based bioinformatics framework to classify metagenomic and genomic sequences with unprecedented accuracy. The distinguishing aspect of MyTaxa is that it employs all genes present in an unknown sequence as classifiers, weighting each gene based on its (predetermined) classifying power at a given taxonomic level and frequency of horizontal gene transfer. MyTaxa also implements a novel classification scheme based on the genome-aggregate average amino acid identity concept to determine the degree of novelty of sequences representing uncharacterized taxa, i.e. whether they represent novel species, genera or phyla. Application of MyTaxa on in silico generated (mock) and real metagenomes of varied read length (100-2000 bp) revealed that it correctly classified at least 5% more sequences than any other tool. The analysis also showed that ∼10% of the assembled sequences from human gut metagenomes represent novel species with no sequenced representatives, several of which were highly abundant in situ such as members of the Prevotella genus. Thus, MyTaxa can find several important applications in microbial identification and diversity studies.

PMID:
24589583
PMCID:
PMC4005636
DOI:
10.1093/nar/gku169
[Indexed for MEDLINE]
Free PMC Article

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