Format

Send to

Choose Destination
See comment in PubMed Commons below
Transbound Emerg Dis. 2013 Nov;60 Suppl 2:137-49. doi: 10.1111/tbed.12150.

Identification and characterization of Theileria annulata heat-shock protein 90 (HSP90) isoforms.

Author information

1
Division of Veterinary Infection-Biology and -Immunology, Department of Infection, Research Center Borstel, Borstel, Schleswig-Holstein, Germany.

Abstract

Heat-shock proteins (HSPs) refer to a group of proteins whose synthesis is enhanced upon sudden increase in temperature or exposure to a variety of other stressors. In this study, Theileria annulata (T. annulata) HSP90 was identified and characterized as a first step to understand the function of this molecule in T. annulata-infected cells. Our results indicated the existence in the genome of T. annulata of two HSP90 genes: one located in chromosome one (TaHSP90-Chr1) and the other in chromosome four (TaHSP90-Chr4). The amino acid alignment between the two isoforms has shown identity and similarity values of 23.52% and 30.26%, respectively. Theileria annulata recombinant HSP90 proteins were expressed using a bacterial expression system and could be recognized in Western blots by rabbit anti-serum raised against an antigenic peptide derived from a unique sequence of TaHSP90-Chr1. On the other hand, bovine HSP90 was detected in T. annulata-infected cells using Western blot and immunocytostaining. To demonstrate the effect of the inhibition of HSP90 on the survival of T. annulata-infected cells, Geldanamycin (GA), a specific inhibitor for HSP90, was used. Upon GA treatment, p53 was observed to translocate into the host cell nucleus, a phenomenon that occurs in cells undergoing apoptosis. Using flowcytometry, a significant increase (P = 0.028) in cell death (%) was observed in T. annulata-infected cells treated with two different GA concentrations, 0.5 and 1 μm, and incubated for 24, 48 and 72 h.

KEYWORDS:

Heat-shock protein 90 (HSP90); P53; Theileria; apoptosis

PMID:
24589114
DOI:
10.1111/tbed.12150
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Wiley
    Loading ...
    Support Center