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Alcohol Clin Exp Res. 2014 May;38(5):1390-400. doi: 10.1111/acer.12378. Epub 2014 Mar 3.

Maternal and neonatal plasma microRNA biomarkers for fetal alcohol exposure in an ovine model.

Author information

1
Department of Neuroscience and Experimental Therapeutics, and Women's Health in Neuroscience Program, Texas A&M Health Science Center, College of Medicine, Bryan, Texas.

Abstract

BACKGROUND:

Plasma or circulating miRNAs (cir miRNAs) have potential diagnostic value as biomarkers for a range of diseases. Based on observations that ethanol (EtOH) altered intracellular miRNAs during development, we tested the hypothesis that plasma miRNAs were biomarkers for maternal alcohol exposure, and for past in utero exposure, in the neonate.

METHODS:

Pregnant sheep were exposed to a binge model of EtOH consumption resulting in an average peak blood alcohol content of 243 mg/dl, for a third-trimester-equivalent period from gestational day 4 (GD4) to GD132. MiRNA profiles were assessed by quantitative PCR analysis in plasma, erythrocyte, and leukocytes obtained from nonpregnant ewes, and plasma from pregnant ewes 24 hours following the last binge EtOH episode, and from newborn lambs, at birth on ~GD147.

RESULTS:

Pregnant ewe and newborn lamb cir miRNA profiles were similar to each other and different from nonpregnant female plasma, erythrocyte, or leukocyte miRNAs. Significant changes in cir miRNA profiles were observed in the EtOH-exposed ewe and, at birth, in the in utero, EtOH-exposed lamb. Cir miRNAs including miR-9, -15b, -19b, and -20a were sensitive and specific measures of EtOH exposure in both pregnant ewe and newborn lamb. Additionally, EtOH exposure altered guide-to-passenger strand cir miRNA ratios in the pregnant ewe, but not in the lamb.

CONCLUSIONS:

Shared profiles between pregnant dam and neonate suggest possible maternal-fetal miRNA transfer. Cir miRNAs are biomarkers for alcohol exposure during pregnancy, in both mother and neonate, and may constitute an important shared endocrine biomarker that is vulnerable to the maternal environment.

KEYWORDS:

Fetal Alcohol Exposure; Maternal Alcohol Exposure; Passenger Strand miRNA; Plasma miRNAs; miRNA*

PMID:
24588274
PMCID:
PMC3999266
DOI:
10.1111/acer.12378
[Indexed for MEDLINE]
Free PMC Article

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