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Biomed Res Int. 2014;2014:951978. doi: 10.1155/2014/951978. Epub 2014 Jan 21.

Assessment of Mycobacterium bovis deleted in p27-p55 virulence operon as candidate vaccine against tuberculosis in animal models.

Author information

1
Instituto de Biotecnología, CICVyA-INTA, N. Repetto and De los Reseros, 1686 Hurlingham, Argentina.
2
Public Health England, Porton Down, Salisbury SP4 0JG, UK.
3
Instituto de Patobiología, CICVyA-INTA, N. Repetto and De los Reseros, 1686 Hurlingham, Argentina.

Abstract

A Mycobacterium bovis knockout in p27-p55 operon was tested as an antituberculosis experimental vaccine in animal models. The mutant MbΔp27-p55 was significantly more attenuated in nude mice than its parental strain but more virulent than BCG Pasteur. Challenge experiments in mice and guinea pigs using M. bovis or M. tuberculosis strains showed similar protection conferred by MbΔp27-p55 mutant than BCG in terms of pathology and bacterial loads in spleen but lower protection than BCG in lungs. When tested in cattle, MbΔp27-p55 did not induce IL-2 expression and induced a very low production of IFNγ, suggesting that the lack of P27/P55 reduces the capacity of M. bovis of triggering an adequate Th1 response.

PMID:
24588000
PMCID:
PMC3918748
DOI:
10.1155/2014/951978
[Indexed for MEDLINE]
Free PMC Article
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