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PLoS One. 2014 Feb 28;9(2):e90505. doi: 10.1371/journal.pone.0090505. eCollection 2014.

Selection for phase variation of LOS biosynthetic genes frequently occurs in progression of non-typeable Haemophilus influenzae infection from the nasopharynx to the middle ear of human patients.

Author information

1
School of Molecular and Microbial Sciences, University of Queensland, St. Lucia, Brisbane, Queensland, Australia.
2
Institute for Glycomics, Griffith University, Gold Coast, Queensland, Australia.
3
Centre for Microbial Pathogenesis, The Research Institute at Nationwide Children's Hospital, Columbus, Ohio, United States of America.

Abstract

Surface structures in Haemophilus influenzae are subject to rapid ON/OFF switching of expression, a process termed phase variation. We analyse tetranucleotide repeats controlling phase variation in lipo-oligosaccharide (LOS) genes of H. influenzae in paired isolates from both the nasopharynx and middle ears of paediatric patients with chronic or recurrent otitis media. A change in expression of at least one of the seven phase variable LOS biosynthesis genes was seen in 12 of the 21 strain pairs. Several strains showed switching of expression in multiple LOS genes, consistent with a key role for phase variable LOS biosynthetic genes in human infection.

PMID:
24587383
PMCID:
PMC3938747
DOI:
10.1371/journal.pone.0090505
[Indexed for MEDLINE]
Free PMC Article

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