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PLoS One. 2014 Feb 19;9(2):e89416. doi: 10.1371/journal.pone.0089416. eCollection 2014.

Conducting polymer electrodes for gel electrophoresis.

Author information

1
Transport and Separations Group, Department of Physics, Chemistry and Biology, Linköping University, Linköping, Sweden.

Abstract

In nearly all cases, electrophoresis in gels is driven via the electrolysis of water at the electrodes, where the process consumes water and produces electrochemical by-products. We have previously demonstrated that π-conjugated polymers such as poly(3,4-ethylenedioxythiophene) (PEDOT) can be placed between traditional metal electrodes and an electrolyte to mitigate electrolysis in liquid (capillary electroosmosis/electrophoresis) systems. In this report, we extend our previous result to gel electrophoresis, and show that electrodes containing PEDOT can be used with a commercial polyacrylamide gel electrophoresis system with minimal impact to the resulting gel image or the ionic transport measured during a separation.

PMID:
24586761
PMCID:
PMC3929695
DOI:
10.1371/journal.pone.0089416
[Indexed for MEDLINE]
Free PMC Article

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