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Nat Methods. 2014 Apr;11(4):410-2. doi: 10.1038/nmeth.2878. Epub 2014 Mar 2.

Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering.

Author information

1
Department of Chemistry, Columbia University, New York, New York, USA.
2
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA.
3
1] Department of Chemistry, Columbia University, New York, New York, USA. [2] Kavli Institute for Brain Science, Columbia University, New York, New York, USA.

Abstract

Sensitive and specific visualization of small biomolecules in living systems is highly challenging. We report stimulated Raman-scattering imaging of alkyne tags as a general strategy for studying a broad spectrum of small biomolecules in live cells and animals. We demonstrate this technique by tracking alkyne-bearing drugs in mouse tissues and visualizing de novo synthesis of DNA, RNA, proteins, phospholipids and triglycerides through metabolic incorporation of alkyne-tagged small precursors.

PMID:
24584195
PMCID:
PMC4040164
DOI:
10.1038/nmeth.2878
[Indexed for MEDLINE]
Free PMC Article

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