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Fermented Pu-erh tea increases in vitro anticancer activities in HT-29 cells and has antiangiogenetic effects on HUVECs.

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Department of Food Science and Nutrition, Pusan National University, Busan 609-735, South Korea; Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, People's Republic of China.
Department of Food Science and Nutrition, Pusan National University, Busan, Korea.
Department of Biotechnology, Research Center on Anti-Obesity and Health Care, Chonnam National University, Yosu, Chonnam, Korea.
Gyeonggi Biocenter, Gyeonggi Institute of Science and Technology Promotion (GSTEP), Suwon, Gyeonggi-do, Korea.


Pu-erh tea is produced in China and known to possess medicinal properties. The anticancer and antiangiogenesis effects of fermented Pu-erh tea on HT-29 colon cancer cells and human umbilical vein endothelial cells, respectively, were examined. Two kinds of unfermented and fermented Pu-erh tea (Seven-son tea cake Pu-erh tea and Xiaguan bowl tea [X]) and green tea were used. An MTT assay showed fermented Pu-erh tea X (85% inhibition) possessed more potent anticancer activities than unfermented Pu-erh tea X (67% inhibition) and green tea (53% inhibition) (P < 0.05). Moreover, fermented Pu-erh tea X increased the number of apoptotic bodies determined through DAPI staining and flow cytometric analysis. Fermented Pu-erh tea X induced apoptosis indicated by increased expression of Bax, caspase-9, and caspase-3 messenger RNA and decreased expression of Bcl-2. Fermented Pu-erh tea X also had an anti-inflammation effect, shown in decreased expression of nuclear factor-κB-p65, inducible nitric oxide synthase, COX-2 messenger RNA and increased expression of IκB-α. Further, fermented Pu-erh teas showed stronger antiangiogenesis effects than the 2 other types of tea. After fermentation, the concentrations of gallic acid, resorcylic acid, quercetin, and kaempferol in Pu-erh tea were increased. These results collectively indicated that fermented and unfermented Pu-erh teas possess stronger anticancer and antiangiogenesis effects than green tea. Furthermore, fermented Pu-erh tea showed stronger functional activities than unfermented Pu-erh tea.

[Indexed for MEDLINE]

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