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Methods. 2014 Jun 15;68(1):82-8. doi: 10.1016/j.ymeth.2014.02.018. Epub 2014 Feb 24.

RNAi screening in Drosophila cells and in vivo.

Author information

1
Drosophila RNAi Screening Center, Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, United States. Electronic address: stephanie_mohr@hms.harvard.edu.

Abstract

Here, I discuss how RNAi screening can be used effectively to uncover gene function. Specifically, I discuss the types of high-throughput assays that can be done in Drosophila cells and in vivo, RNAi reagent design and available reagent collections, automated screen pipelines, analysis of screen results, and approaches to RNAi results verification.

KEYWORDS:

Cell-based assays; Drosophila; Functional genomics; High-throughput screening; In vivo assays; RNAi

PMID:
24576618
PMCID:
PMC4206080
DOI:
10.1016/j.ymeth.2014.02.018
[Indexed for MEDLINE]
Free PMC Article

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