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Biomed Opt Express. 2014 Jan 27;5(2):609-20. doi: 10.1364/BOE.5.000609. eCollection 2014 Feb 1.

Characterization of eosinophilic esophagitis murine models using optical coherence tomography.

Author information

1
Department of Electrical and Computer Engineering, Lehigh University, Bethlehem, PA-18015, USA ; Center for Photonics and Nanoelectronics, Lehigh University, Bethlehem, PA-18015, USA.
2
Department of Microbiology University of Pennsylvania, Philadelphia, PA 19104, USA ; Institute for Immunology, Perelman School of Medicine University of Pennsylvania, Philadelphia, PA 19104, USA ; Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
3
Department of Electrical and Computer Engineering, Lehigh University, Bethlehem, PA-18015, USA ; Center for Photonics and Nanoelectronics, Lehigh University, Bethlehem, PA-18015, USA ; Bioengineering Program, Lehigh University, Bethlehem, PA-18015, USA.

Abstract

Pre-clinical studies using murine models are critical for understanding the pathophysiological mechanisms underlying immune-mediated disorders such as Eosinophilic esophagitis (EoE). In this study, an optical coherence tomography (OCT) system capable of providing three-dimensional images with axial and transverse resolutions of 5 µm and 10 µm, respectively, was utilized to obtain esophageal images from a murine model of EoE-like disease ex vivo. Structural changes in the esophagus of wild-type (Tslpr(+/+) ) and mutant (Tslpr(-/-) ) mice with EoE-like disease were quantitatively evaluated and food impaction sites in the esophagus of diseased mice were monitored using OCT. Here, the capability of OCT as a label-free imaging tool devoid of tissue-processing artifacts to effectively characterize murine EoE-like disease models has been demonstrated.

KEYWORDS:

(110.4500) Optical coherence tomography; (110.6880) Three-dimensional image acquisition; (170.0110) Imaging systems; (170.2680) Gastrointestinal; (170.3880) Medical and biological imaging; (170.6935) Tissue characterization

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