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Gene Ther. 2014 Apr;21(4):444-9. doi: 10.1038/gt.2014.5. Epub 2014 Feb 27.

Efficient delivery of lentiviral vectors into resting human CD4 T cells.

Author information

1
Virology & Immunology, J. David Gladstone Institutes, San Francisco, CA, USA.
2
1] Virology & Immunology, J. David Gladstone Institutes, San Francisco, CA, USA [2] Department of Medicine, University of California, San Francisco, San Francisco, CA, USA [3] Department of Microbiology and Immunology, University of California, San Francisco, CA, USA.

Abstract

Resting human CD4 T cells are highly resistant to transfection or infection with lentiviral vectors derived from the human immunodeficiency virus. We now describe a flexible and efficient approach involving virus-like particles containing simian immunodeficiency virus lentiviral gene product protein X and pseudotyping with CXCR4-tropic HIV Env. This method permits effective genetic manipulation of these cells while preserving their naturally quiescent state. This technology can also be extended to primary lymphoid cultures where authentic cellular composition and functional relationships are preserved.

PMID:
24572792
PMCID:
PMC4006095
DOI:
10.1038/gt.2014.5
[Indexed for MEDLINE]
Free PMC Article
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