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Nature. 2014 Mar 20;507(7492):366-370. doi: 10.1038/nature12979. Epub 2014 Feb 23.

IL-35-producing B cells are critical regulators of immunity during autoimmune and infectious diseases.

Author information

1
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute, Charitéplatz 1, 10117 Berlin, Germany.
2
University of Edinburgh, Centre for Inflammation Research and Centre for Multiple Sclerosis Research, Queen's Medical Research Institute, Edinburgh, EH16 4TJ, United Kingdom.
3
Charité Universitätsmedizin Berlin, CC12, Dept. Medicine/Rheumatology and Clinical Immunology, 10117 Berlin, Germany.
4
Neuroimmunology Unit, Montreal Neurological Institute and Hospital, McGill University, Montreal, Quebec, H3A2B4, Canada.
5
Virologie et Immunologie Moléculaires, INRA, 78352 Jouy-en-Josas, France.
6
Medical Clinic 1, Kussmaul Campus for Medical Research, University of Erlangen-Nürnberg, Germany.
7
Institut für Klinische Neuroimmunologie Klinikum der Ludwig-Maximilians-Universität München, 81377 München, Germany.
8
Immunpathologie, Research Center ImmunoSciences, 12203 Berlin, Germany.
9
Max Planck Institute of Infection Biology, Department of Immunology, Charitéplatz 1, 10117 Berlin, Germany.
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Contributed equally

Abstract

B lymphocytes have critical roles as positive and negative regulators of immunity. Their inhibitory function has been associated primarily with interleukin 10 (IL-10) because B-cell-derived IL-10 can protect against autoimmune disease and increase susceptibility to pathogens. Here we identify IL-35-producing B cells as key players in the negative regulation of immunity. Mice in which only B cells did not express IL-35 lost their ability to recover from the T-cell-mediated demyelinating autoimmune disease experimental autoimmune encephalomyelitis (EAE). In contrast, these mice displayed a markedly improved resistance to infection with the intracellular bacterial pathogen Salmonella enterica serovar Typhimurium as shown by their superior containment of the bacterial growth and their prolonged survival after primary infection, and upon secondary challenge, compared to control mice. The increased immunity found in mice lacking IL-35 production by B cells was associated with a higher activation of macrophages and inflammatory T cells, as well as an increased function of B cells as antigen-presenting cells (APCs). During Salmonella infection, IL-35- and IL-10-producing B cells corresponded to two largely distinct sets of surface-IgM(+)CD138(hi)TACI(+)CXCR4(+)CD1d(int)Tim1(int) plasma cells expressing the transcription factor Blimp1 (also known as Prdm1). During EAE, CD138(+) plasma cells were also the main source of B-cell-derived IL-35 and IL-10. Collectively, our data show the importance of IL-35-producing B cells in regulation of immunity and highlight IL-35 production by B cells as a potential therapeutic target for autoimmune and infectious diseases. This study reveals the central role of activated B cells, particularly plasma cells, and their production of cytokines in the regulation of immune responses in health and disease.

PMID:
24572363
PMCID:
PMC4260166
DOI:
10.1038/nature12979
[Indexed for MEDLINE]
Free PMC Article

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