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Heredity (Edinb). 2014 May;112(5):508-18. doi: 10.1038/hdy.2013.133. Epub 2014 Feb 26.

Fine-mapping quantitative trait loci affecting murine external ear tissue regeneration in the LG/J by SM/J advanced intercross line.

Author information

1
Department of Anatomy and Neurobiology, Washington University School of Medicine, St Louis, MO, USA.
2
Molecular and Cellular Oncogenesis, The Wistar Institute, Philadelphia, PA, USA.
3
Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, PA, USA.

Abstract

External ear hole closure in LG/J mice represents a model of regenerative response. It is accompanied by the formation of a blastema-like structure and the re-growth of multiple tissues, including cartilage. The ability to regenerate tissue is heritable. An F34 advanced intercross line of mice (Wustl:LG,SM-G34) was generated to identify genomic loci involved in ear hole closure over a 30-day healing period. We mapped 19 quantitative trait loci (QTL) for ear hole closure. Individual gene effects are relatively small (0.08 mm), and most loci have co-dominant effects with phenotypically intermediate heterozygotes. QTL support regions were limited to a median size of 2 Mb containing a median of 19 genes. Positional candidate genes were evaluated using differential transcript expression between LG/J and SM/J healing tissue, function analysis and bioinformatic analysis of single-nucleotide polymorphisms in and around positional candidate genes of interest. Analysis of the set of 34 positional candidate genes and those displaying expression differences revealed over-representation of genes involved in cell cycle regulation/DNA damage, cell migration and adhesion, developmentally related genes and metabolism. This indicates that the healing phenotype in LG/J mice involves multiple physiological mechanisms.

PMID:
24569637
PMCID:
PMC3998788
DOI:
10.1038/hdy.2013.133
[Indexed for MEDLINE]
Free PMC Article

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