Format

Send to

Choose Destination
See comment in PubMed Commons below
J Ethnopharmacol. 2014 May 14;153(3):922-7. doi: 10.1016/j.jep.2014.02.029. Epub 2014 Feb 23.

A herbal formula consisting of Rosae Multiflorae Fructus and Lonicerae Japonicae Flos inhibits inflammatory mediators in LPS-stimulated RAW 264.7 macrophages.

Author information

  • 1Centre for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong.
  • 2Shenzhen Institute of Integrative Medicine, Shenzhen Second People׳s Hospital, Shenzhen, China.
  • 3Centre for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong. Electronic address: zlyu@hkbu.edu.hk.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

A herbal formula (RL) consisting of Rosae Multiflorae Fructus (Yingshi) and Lonicerae Japonicae Flos (Jinyinhua) has been traditionally used to treat inflammatory disorders. This study aims to investigate the anti-inflammatory mode and mechanism of action of the ethanol extract of RL so as to provide a pharmacological basis for the use of RL in treating inflammatory diseases.

MATERIALS AND METHOD:

RL consisting of Yingshi and Jinyinhua (in 5:3 ratio) was extracted using absolute ethanol. We investigated its effects on nitric oxide (NO), interleukin-6 (IL-6), tumour necrosis factor (TNF)-α, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinases (MAPKs) and nuclear factor κB (NFκB) in mouse RAW 264.7 macrophages activated with lipopolysaccharide (LPS).

RESULTS:

RL could decrease the secretion of NO, IL-6 and TNF-α into the culture medium and the cellular protein levels of iNOS and COX-2, which were associated with the reduction of the phosphorylation/activation of JNK and p38, and the inhibition of the transcriptional activity of NF-κB.

CONCLUSIONS:

The present study demonstrated an inhibitory effect of RL on the inflammatory mediators regulated by the NF-κB and MAPK signalling pathways in LPS-stimulated RAW 264.7 macrophages, providing a pharmacological basis for RL in the control of inflammatory disorders.

KEYWORDS:

Anti-inflammation; Chlorogenic acid (PubChem CID: 1794427); Gallic acid (PubChem CID: 370); Lipopolysaccharide (PubChem CID: 11970143); Lonicera japonica; MAPK; NF-κB; Rosa multiflora

PMID:
24568773
DOI:
10.1016/j.jep.2014.02.029
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center