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Front Genet. 2014 Feb 6;5:1. doi: 10.3389/fgene.2014.00001. eCollection 2014.

Cytoplasmic 5'-3' exonuclease Xrn1p is also a genome-wide transcription factor in yeast.

Author information

1
Departamento de Bioquímica y Biología Molecular and ERI Biotecmed, Universitat de València Burjassot, Spain.
2
Departamento de Genética and Instituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla Seville, Spain.
3
Faculty of Medicine, Department of Molecular Microbiology, Technion-Israel Institute of Technology Haifa, Israel.

Abstract

The 5' to 3' exoribonuclease Xrn1 is a large protein involved in cytoplasmatic mRNA degradation as a critical component of the major decaysome. Its deletion in the yeast Saccharomyces cerevisiae is not lethal, but it has multiple physiological effects. In a previous study, our group showed that deletion of all tested components of the yeast major decaysome, including XRN1, results in a decrease in the synthetic rate and an increase in half-life of most mRNAs in a compensatory manner. Furthermore, the same study showed that the all tested decaysome components are also nuclear proteins that bind to the 5' region of a number of genes. In the present work, we show that disruption of Xrn1 activity preferentially affects both the synthesis and decay of a distinct subpopulation of mRNAs. The most affected mRNAs are the transcripts of the highly transcribed genes, mainly those encoding ribosome biogenesis and translation factors. Previously, we proposed that synthegradases play a key role in regulating both mRNA synthesis and degradation. Evidently, Xrn1 functions as a synthegradase, whose selectivity might help coordinating the expression of the protein synthetic machinery. We propose to name the most affected genes "Xrn1 synthegradon."

KEYWORDS:

mRNA decay; mRNA stability; mRNA synthesis; nascent transcription; transcription rate

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