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Nutrients. 2014 Feb 21;6(2):776-89. doi: 10.3390/nu6020776.

Characterization of oilseed lipids from "DHA-producing Camelina sativa": a new transformed land plant containing long-chain omega-3 oils.

Author information

1
CSIRO Food Futures Flagship, Marine and Atmospheric Research, Castray Esplanade Hobart, Tasmania 7000, Australia. Peter.Mansour@csiro.au.
2
CSIRO Food Futures Flagship, Plant Industry, P.O. Box 1600, Canberra ACT 2601, Australia. Pushkar.Shrestha@csiro.au.
3
CSIRO Food Futures Flagship, Plant Industry, P.O. Box 1600, Canberra ACT 2601, Australia. Srinivas.Belide@csiro.au.
4
CSIRO Food Futures Flagship, Plant Industry, P.O. Box 1600, Canberra ACT 2601, Australia. James.Petrie@csiro.au.
5
CSIRO Food Futures Flagship, Marine and Atmospheric Research, Castray Esplanade Hobart, Tasmania 7000, Australia. Peter.Nichols@csiro.au.
6
CSIRO Food Futures Flagship, Plant Industry, P.O. Box 1600, Canberra ACT 2601, Australia. Surinder.Singh@csiro.au.

Abstract

New and sustainable sources of long-chain (LC, ≥C₂₀) omega-3 oils containing DHA (docosahexaenoic acid, 22:6ω3) are required to meet increasing demands. The lipid content of the oilseed of a novel transgenic, DHA-producing land plant, Camelina sativa, containing microalgal genes able to produce LC omega-3 oils, contained 36% lipid by weight with triacylglycerols (TAG) as the major lipid class in hexane extracts (96% of total lipid). Subsequent chloroform-methanol (CM) extraction recovered further lipid (~50% polar lipid, comprising glycolipids and phospholipids) and residual TAG. The main phospholipid species were phosphatidyl choline and phosphatidyl ethanolamine. The % DHA was: 6.8% (of total fatty acids) in the TAG-rich hexane extract and 4.2% in the polar lipid-rich CM extract. The relative level of ALA (α-linolenic acid, 18:3ω3) in DHA-camelina seed was higher than the control. Major sterols in both DHA- and control camelina seeds were: sitosterol, campesterol, cholesterol, brassicasterol and isofucosterol. C₁₆-C₂₂ fatty alcohols, including iso-branched and odd-chain alcohols were present, including high levels of iso-17:0, 17:0 and 19:0. Other alcohols present were: 16:0, iso-18:0, 18:0 and 18:1 and the proportions varied between the hexane and CM extracts. These iso-branched odd-chain fatty alcohols, to our knowledge, have not been previously reported. These components may be derived from wax esters, or free fatty alcohols.

PMID:
24566436
PMCID:
PMC3942731
DOI:
10.3390/nu6020776
[Indexed for MEDLINE]
Free PMC Article
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