Format

Send to

Choose Destination
Proc Natl Acad Sci U S A. 1988 Aug;85(15):5738-42.

Isolation of cDNAs of scrapie-modulated RNAs by subtractive hybridization of a cDNA library.

Author information

1
GRECC, Edith Nourse Rogers Memorial Veterans Administration Hospital, Bedford, MA 01730.

Abstract

We have developed a subtractive cloning procedure based on the hybridization of single-stranded cDNA libraries constructed in pi H3M, a vector containing the phage M13 origin of replication. We have used this strategy to isolate three transcripts whose abundance is increased in scrapie-infected brain. DNA sequence analysis showed that they represent glial fibrillary acidic protein, metallothionein II, and the B chain of alpha-crystallin; the latter two may represent a response to stress.

PMID:
2456582
PMCID:
PMC281836
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center