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J Antimicrob Chemother. 2014 Sep;69(9):2383-7. doi: 10.1093/jac/dku045. Epub 2014 Feb 20.

Molecular epidemiology of Acinetobacter baumannii in Iran: endemic and epidemic spread of multiresistant isolates.

Author information

1
Department of Microbiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
2
Department of Microbiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Department of Sciences for Health Promotion and Mother-Child Care 'G. D.'Alessandro', University of Palermo, Palermo, Italy.
3
Department of Sciences for Health Promotion and Mother-Child Care 'G. D.'Alessandro', University of Palermo, Palermo, Italy.
4
Department of Sciences for Health Promotion and Mother-Child Care 'G. D.'Alessandro', University of Palermo, Palermo, Italy caterina.mammina@unipa.it.

Abstract

OBJECTIVES:

We examined the molecular epidemiology of Acinetobacter baumannii clinical isolates from two cities (Tehran and Tabriz) of Iran.

METHODS:

DiversiLab repetitive extragenic palindromic PCR (rep-PCR), multilocus sequence typing and sequence group multiplex PCR were performed. The presence of resistance mechanisms including metallo-β-lactamases, extended-spectrum β-lactamases, OXA carbapenemases, aminoglycoside-modifying enzymes and RNA methylases was also investigated.

RESULTS:

DiversiLab rep-PCR identified 11 clusters and 11 singleton isolates. Twelve sequence types (STs), including six novel types, were identified. Sequence groups (SGs) 1-3 as well as five additional banding patterns were detected by multiplex PCR. A local outbreak in a general hospital in Tabriz with an SG1/ST2 profile was identified. Isolates of international clone II showed the highest prevalence and the most heterogeneous combination of resistance determinants.

CONCLUSIONS:

Several different multiresistant strains of A. baumannii were shown to circulate in Iran. The selection and spread of the SG1/ST2 clone might have been favoured by the acquisition of resistance genes in the absence of adequate infection control measures.

KEYWORDS:

DiversiLab rep-PCR; MLST; multidrug resistance

PMID:
24562615
DOI:
10.1093/jac/dku045
[Indexed for MEDLINE]

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