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Methods. 2014 Nov;70(1):12-9. doi: 10.1016/j.ymeth.2014.01.022. Epub 2014 Feb 21.

Tissue fixation and the effect of molecular fixatives on downstream staining procedures.

Author information

1
Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Cambridge CB2 0RE, UK. Electronic address: will.howat@cruk.cam.ac.uk.
2
Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Cambridge CB2 0RE, UK.

Abstract

It is impossible to underplay the importance of fixation in histopathology. Whether the scientist is interested in the extraction of information on lipids, proteins, RNA or DNA, fixation is critical to this extraction. This review aims to give a brief overview of the current "state of play" in fixation and focus on the effect fixation, and particularly the effect of the newer brand of "molecular fixatives" have on morphology, histochemistry, immunohistochemistry and RNA/DNA analysis. A methodology incorporating the creation of a fixation tissue microarray for the study of the effect of fixation on histochemistry is detailed.

KEYWORDS:

Histochemistry; Molecular fixatives; Tissue fixation; Tissue microarray

PMID:
24561827
PMCID:
PMC4240801
DOI:
10.1016/j.ymeth.2014.01.022
[Indexed for MEDLINE]
Free PMC Article

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