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Methods Cell Biol. 2014;121:49-60. doi: 10.1016/B978-0-12-800281-0.00004-X.

The facile generation of two-dimensional stiffness maps in durotactic cell platforms through thickness projections of three-dimensional submerged topography.

Author information

1
Biological and Soft Systems, Cavendish Laboratory, University of Cambridge, Cambridge, United Kingdom.
2
Biological and Soft Systems, Cavendish Laboratory, University of Cambridge, Cambridge, United Kingdom; Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.
3
Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary; Institute for Integrated Cell-Material Sciences, Kyoto University, Kyoto, Japan.
4
Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.
5
Biological and Soft Systems, Cavendish Laboratory, University of Cambridge, Cambridge, United Kingdom; Institute for Integrated Cell-Material Sciences, Kyoto University, Kyoto, Japan.

Abstract

An innovative platform that aims to facilitate studies of how adherent cells migrate in response to rigidity gradients or durotaxis has been developed. Soft polyacrylamide gel-based cell culture scaffolds are used to fabricate flat surfaces containing elasticity gradients through changes in the underlying patterned features. Moreover, this inert gel surface supports long-term cell viability and offers a tunable stiffness. By manipulating the thickness of the gel substrate through the embedded patterns, this system is also capable of directing collective cell patterning.

KEYWORDS:

Cell migration; Durotaxis; Mechanosensing; Scaffolds

[Indexed for MEDLINE]

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