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Zhong Yao Cai. 2013 Aug;36(8):1215-8.

[Genetic diversity analysis of Schisandra sphenanthera in Qinling Mountains based on SSR markers].

[Article in Chinese]

Author information

1
Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, Ministry of Education,China. jieyuan@stu.snnu.edu.cn
2
National Engineering Laboratory for Resource Development of Endangered Chinese Crude Drugs in Northwest of China, China.
3
Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, Ministry of Education,China.

Abstract

OBJECTIVE:

To analyse the genetic diversity of wild Schisandra sphenanthera in Qinling Mountains.

METHODS:

The genetic diversity of wild S. sphenanthera was investigated by SSR (Simple Sequences Repeats) markers.

RESULTS:

Nine pair primers generated a total of 61 bands 53 of which were polymorphic, and 6.8 genotypes were detected by each SSR primer pair on an average ranging from 5 to 11. The percentage of polymorphic band was 86.88%. The coefficient of genetic differentiation (G(st) = 0.3524) showed great genetic variation occurred within populations. Gene flow (N(m)) was 1.1850, which showed that gene flow occurred among populations. The highest genetic diversity of five populations was Yangxian population. And cluster analysis was based on genetic similarity coefficient. The five populations of wild S. sphenanthera samples were classified into two large groups, group I included Pingli population, Shanyang population and Ningqiang population; Group II included Taibai population and Yangxian population.

CONCLUSION:

The S. sphenanthera in Qinling Mountains, especially the regions of Hanzhong,Baoji and Shangluo in Shaanxi province, reveals high genetic diversity, which benefits the breeding of new varieties. It is necessary to protect the resource and ensure the sustainable development and utilization of it.

PMID:
24558815
[Indexed for MEDLINE]
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