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PLoS One. 2014 Feb 18;9(2):e89100. doi: 10.1371/journal.pone.0089100. eCollection 2014.

Discrimination of wild Paris based on near infrared spectroscopy and high performance liquid chromatography combined with multivariate analysis.

Author information

  • 1Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan, the People's Republic of China.
  • 2Yunnan Reascend Tobacco Technology (Group) Co., Ltd., Kunming, Yunnan, the People's Republic of China.
  • 3College of Resources and Environment, Yuxi Normal University, Yuxi, Yunnan, the People's Republic of China.

Abstract

Different geographical origins and species of Paris obtained from southwestern China were discriminated by near infrared (NIR) spectroscopy and high performance liquid chromatography (HPLC) combined with multivariate analysis. The NIR parameter settings were scanning (64 times), resolution (4 cm(-1)), scanning range (10,000 cm(-1)∼4000 cm(-1)) and parallel collection (3 times). NIR spectrum was optimized by TQ 8.6 software, and the ranges 7455∼6852 cm(-1) and 5973∼4007 cm(-1) were selected according to the spectrum standard deviation. The contents of polyphyllin I, polyphyllin II, polyphyllin VI, and polyphyllin VII and total steroid saponins were detected by HPLC. The contents of chemical components data matrix and spectrum data matrix were integrated and analyzed by partial least squares discriminant analysis (PLS-DA). From the PLS-DA model of NIR spectrum, Paris samples were separated into three groups according to the different geographical origins. The R(2)X and Q(2)Y described accumulative contribution rates were 99.50% and 94.03% of the total variance, respectively. The PLS-DA model according to 12 species of Paris described 99.62% of the variation in X and predicted 95.23% in Y. The results of the contents of chemical components described differences among collections quantitatively. A multivariate statistical model of PLS-DA showed geographical origins of Paris had a much greater influence on Paris compared with species. NIR and HPLC combined with multivariate analysis could discriminate different geographical origins and different species. The quality of Paris showed regional dependence.

PMID:
24558477
PMCID:
PMC3928364
DOI:
10.1371/journal.pone.0089100
[PubMed - indexed for MEDLINE]
Free PMC Article
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