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Methods Mol Biol. 2014;1114:269-77. doi: 10.1007/978-1-62703-761-7_17.

RNA-guided genome editing of mammalian cells.

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1
Broad Institute of MIT and Harvard, Massachusetts Institute of Technology, Cambridge, USA.

Abstract

The microbial CRISPR-Cas adaptive immune system can be harnessed to facilitate genome editing in eukaryotic cells (Cong L et al., Science 339, 819-823, 2013; Mali P et al., Science 339, 823-826, 2013). Here we describe a protocol for the use of the RNA-guided Cas9 nuclease from the Streptococcus pyogenes type II CRISPR system to achieve specific, scalable, and cost-efficient genome editing in mammalian cells.

PMID:
24557909
DOI:
10.1007/978-1-62703-761-7_17
[Indexed for MEDLINE]
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