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Anal Biochem. 1988 Apr;170(1):183-5.

A stain for iron-containing proteins sensitive to nanogram levels of iron.

Author information

1
Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710.

Abstract

A method for staining iron proteins on polyacrylamide gel electropherograms is described. It is based upon catalysis, by iron, of the oxidation of diaminobenzoic acid by hydrogen peroxide. Bands containing as little as 5 ng of protein-bound iron can be visualized by this method.

PMID:
2455457
DOI:
10.1016/0003-2697(88)90106-6
[Indexed for MEDLINE]

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