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Clin Microbiol Infect. 2014 Jul;20(7):O456-9. doi: 10.1111/1469-0691.12446. Epub 2014 Feb 19.

Bacteriophages as a reservoir of extended-spectrum β-lactamase and fluoroquinolone resistance genes in the environment.

Author information

1
Catalan Institute for Water Research (ICRA), Scientific and Technological Park of the University of Girona, Girona, Spain.

Abstract

Six antibiotic resistance genes (blaCTX-M , blaSHV , blaTEM , qnrA, qnrB and qnrS) were quantified by qPCR in both phage and bacterial DNA fractions of environmental water samples in order to determine the contribution of phages to the dissemination of antibiotic resistance genes (ARGs) in the environment. Although the highest copy numbers (p <0.05) of ARGs were detected in the bacterial DNA fraction, qnrS and blaSHV genes were found in the phage DNA from all samples analysed, reaching up to 4 log10 copy numbers/mL in hospital samples. These results indicate that bacteriophages are a potential reservoir of resistance genes and may act as efficient vehicles for horizontal gene transfer.

KEYWORDS:

Antibiotic resistance genes; bacteriophages; hospital and wastewater treatment plant effluents; public health

PMID:
24552593
DOI:
10.1111/1469-0691.12446
[Indexed for MEDLINE]
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