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Int J Clin Exp Pathol. 2014 Jan 15;7(2):709-15. eCollection 2014.

Activation of the IL-6/JAK/STAT3 signaling pathway in human middle ear cholesteatoma epithelium.

Author information

1
Department of Otolaryngology Head and Neck Surgery, The Second Xiangya Hospital of Central South University Changsha, China.
2
Department of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital of Wenzhou Medical University Wenzhou, Zhejiang, China.
3
Department of Human Anatomy, University of South China Hengyang, China.

Abstract

Interleukin-6 (IL-6) is one of the most important cytokines which has been shown to play a critical role in the pathogenesis of cholesteatoma. In this study, we aimed to investigate the expression of interleukin-6 (IL-6) and phosphorylated signal transducer and activator of transcription 3 (p-STAT3) in middle ear cholesteatoma epithelium in an effort to determine the role of IL-6/JAK/STAT3 signaling pathway in the pathogenesis of cholesteatoma. Immunohistochemistry was used to examine the expression of IL-6 and p-STAT3 in 25 human middle ear cholesteatoma samples and 15 normal external auditory canal (EAC) epithelium specimens. We also analyzed the relation of IL-6 and p-STAT3 expression levels to the degree of bone destruction in cholesteatoma. We found that the expression of IL-6 and p-STAT3 were significantly higher in cholesteatoma epithelium than in normal EAC epithelium (p<0.05). In cholesteatoma epithelium, a significant positive association was observed between IL-6 and p-STAT3 expression (p<0.05). However, no significant relationships were observed between the degree of bone destruction and the levels of IL-6 and p-STAT3 expression (p>0.05). To conclude, our results support the concept that IL-6/JAK/STAT3 signaling pathway is active and may play an important role in the mechanisms of epithelial hyper-proliferation responsible for cholesteatoma.

KEYWORDS:

Cholesteatoma; IL-6; STAT3

PMID:
24551293
PMCID:
PMC3925917
[Indexed for MEDLINE]
Free PMC Article

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