Format

Send to

Choose Destination
PLoS One. 2014 Feb 14;9(2):e89047. doi: 10.1371/journal.pone.0089047. eCollection 2014.

Endothelial cell proliferation in swine experimental aneurysm after coil embolization.

Author information

1
Department of Neurosurgery, Juntendo University School of Medicine, Tokyo, Japan.
2
Department of Neurosurgery, Juntendo University Urayasu Hospital, Chiba, Japan.
3
Department of Neurology, Juntendo University Urayasu Hospital, Chiba, Japan.
4
Department of Neurosurgery, Juntendo University School of Medicine, Tokyo, Japan ; Department of Neuroendovascular Therapy, Juntendo University School of Medicine, Tokyo, Japan.

Abstract

After coil embolization, recanalization in cerebral aneurysms adversely influences long-term prognosis. Proliferation of endothelial cells on the coil surface may reduce the incidence of recanalization and further improve outcomes after coil embolization. We aimed to map the expression of proliferating tissue over the aneurysmal orifice and define the temporal profile of tissue growth in a swine experimental aneurysm model. We compared the outcomes after spontaneous thrombosis with those of coil embolization using histological and morphological techniques. In aneurysms that we not coiled, spontaneous thrombosis was observed, and weak, easily detachable proliferating tissue was evident in the aneurysmal neck. In contrast, in the coil embolization group, histological analysis showed endothelial-like cells lining the aneurysmal opening. Moreover, immunohistochemical and morphological analysis suggested that these cells were immature endothelial cells. Our results indicated the existence of endothelial cell proliferation 1 week after coil embolization and showed immature endothelial cells in septal tissue between the systemic circulation and the aneurysm. These findings suggest that endothelial cells are lead to and proliferate in the former aneurysmal orifice. This is the first examination to evaluate the temporal change of proliferating tissue in a swine experimental aneurysm model.

PMID:
24551215
PMCID:
PMC3925181
DOI:
10.1371/journal.pone.0089047
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Public Library of Science Icon for PubMed Central
Loading ...
Support Center