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J Nucl Med. 2014 Mar;55(3):446-51. doi: 10.2967/jnumed.113.129619. Epub 2014 Feb 18.

Multimodal molecular imaging of integrin αvβ3 for in vivo detection of pancreatic cancer.

Author information

1
II. Medizinische Klinik, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.

Abstract

Pancreatic ductal adenocarcinoma (PDAC) is a lethal disease. Late detection of then nonresectable or metastasized tumors emphasizes the need for novel imaging approaches. Here, we report on so far nonexploited potentials of αvβ3 integrin-targeted molecular imaging technologies for detection of PDAC using genetically engineered mouse models.

METHODS:

Immunohistochemistry and Western blot were used for characterization of αvβ3 expression in murine and human PDAC. We applied IntegriSense 680 fluorescence molecular tomography, intraoperative fluorescence imaging, and (68)Ga-NODAGA-RGD PET for αvβ3 integrin molecular in vivo imaging of spontaneous PDAC occurring in Ptf1a(+/Cre);Kras(+/LSL-G12D);p53(LoxP/LoxP) mice. (NODAGA is 1,4,7-triazacyclononane-1,4-bis[acetic acid]-7-[2-glutaric acid] and RGD is arginine-glycine-aspartic acid.)

RESULTS:

αvβ3 integrin is expressed in tumor cells of human and murine PDAC. IntegriSense fluorescence molecular tomography and (68)Ga-NODAGA-RGD PET enabled faithful visualization of PDAC. Furthermore, intraoperative optical imaging with IntegriSense 680 allowed good delineation of tumor borders.

CONCLUSION:

Imaging approaches targeting αvβ3 integrin expand the potential of molecular imaging for identification of αvβ3-positive PDAC with potential implications in early detection, fluorescence-guided surgery, and therapy monitoring.

KEYWORDS:

fluorescence molecular tomography; genetically engineered mice; integrin αvβ3; pancreatic cancer; positron emission tomography

PMID:
24549287
DOI:
10.2967/jnumed.113.129619
[Indexed for MEDLINE]
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