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J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Mar 1;951-952:119-28. doi: 10.1016/j.jchromb.2014.01.011. Epub 2014 Jan 29.

A versatile ultra-high performance LC-MS method for lipid profiling.

Author information

1
Institute of Molecular Biosciences, University of Graz, Humboldtstraße 50/II, 8010 Graz, Austria.
2
Institute of Molecular Biosciences, University of Graz, Humboldtstraße 50/II, 8010 Graz, Austria; Omics Center Graz, Austria. Electronic address: gerald.rechberger@uni-graz.at.

Abstract

A new UPLC-based untargeted lipidomic approach using a qTOF hybrid mass spectrometer is introduced. The applied binary gradient enables separations of lipid species including constitutional isomeric compounds and low abundant lipid classes such as phosphatidic acid (PA). Addition of phosphoric acid to the solvents improves peak shapes for acidic phospholipids. MS(E) scans allow simultaneous acquisition of full scan data and collision induced fragmentation to improve identification of lipid classes and to obtain structural information. The method was used to investigate the lipidome of yeast.

KEYWORDS:

Glycerophospholipids; Lipidomics; Mass spectrometry; Neutral lipids; Ultra-performance liquid chromatography

PMID:
24548922
PMCID:
PMC3946075
DOI:
10.1016/j.jchromb.2014.01.011
[Indexed for MEDLINE]
Free PMC Article

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