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Zhonghua Yi Xue Za Zhi. 2013 Dec 17;93(47):3801-3.

[Study of the expression and function of Syk and VEGF-C in the development of non-small cell lung cancer].

[Article in Chinese]

Author information

1
Department of Surgery, the Second Hospital of Shandong University,Jinan 250033, China.
2
Department of Surgery, the Second Hospital of Shandong University,Jinan 250033, China. Email:menglin2002@163.com.

Abstract

OBJECTIVE:

To explore the role of Syk and VEGF-C in the development of NSCLC.

METHODS:

Transfered these genes(eukaryotic expression vector pcDNA3.1-VEGF-C and pLNCX-syk)into A549 cells with the liposomes. Tested the expression of VEGF-C mRNA and Syk mRNA with RT-PCR.Investigated the cell invasion assay with transwell chamber in vitro. Analysis the expression of VEGF-C proteins in A549 cells and detect Syk and VEGF-C proteins of 81 NSCLC tissue samples with immunohistochemical staining.

RESULTS:

Higher expression of VEGF-C was revealed in VEGF-C-construct-transfected A549 cell than that in controls through RT-PCR (P < 0.05) and immunohistochemistry(P < 0.01).RT-PCR also revealed that Syk expression was higher in Syk-construct-transfected cells than in controls (P < 0.05). The cell invading experiments showed that there was more invaded cells in both transfected terms than in controls (P < 0.05). The expression of Syk protein in NSCLC tissue were significantly lower than that in the normal lung tissue (P < 0.05), while the expression of VEGF-C protein in NSCLC tissue were significantly higher than that in the normal lung tissue(P < 0.05). The expression of Syk and VEGF-C has a negative correlationship (r = -1.000, P = 0.019).

CONCLUSION:

The expression of Syk and VEGF-C has a negative correlationship in NSCLC tissue, VEGF-C-construct-transfected A549 cells are more aggressive than Syk-construct-transfected cells. And they may cooperated with each other in the development of NSCLC.

PMID:
24548403
[Indexed for MEDLINE]

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