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Mol Med Rep. 2014 Apr;9(4):1388-94. doi: 10.3892/mmr.2014.1941. Epub 2014 Feb 10.

Anti-inflammatory activity of a methanol extract from Ardisia tinctoria on mouse macrophages and paw edema.

Author information

1
Biomolecular Science, University of Science and Technology, Daejeon 305‑806, Republic of Korea.
2
Department of Biotechnology, Korea University, Seoul 136‑713, Republic of Korea.
3
Department of Toxicology, College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea.
4
Department of Botany, Institute of Ecology and Biological Resources,Vietnam Academy of Science and Technology, Hanoi 10307, Vietnam.
5
Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungcheongbuk 363-883, Republic of Korea.

Abstract

Ardisia tinctoria (AT) is a plant of the Myrsinaceae family. No studies on its anti-inflammatory effects have yet been reported. This study investigated the anti-inflammatory activity of AT. A non-cytotoxic methanol extract of AT inhibited the expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2), leading to significantly reduced levels of nitric oxide (NO) and prostaglandin E2 (PGE2) and of two proteins regulated by these, interleukin-1β (IL-1β) and IL-6, in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. The thickness of paw edema induced in vivo in mice by carrageenan administration was effectively reduced by the AT extract. Translocation of the nuclear factor-κB (NF-κB) subunit 65 (p65) into the nucleus and phosphorylation of mitogen-activated protein kinase kinase (MEK) and extracellular signal-related kinase (ERK) were inhibited by the AT extract. Our results indicated that a methanol extract of AT downregulates the inflammatory response by blocking phosphorylation of MEK and ERK and activation of NF-κB. To the best of our knowledge, this is the first study of anti-inflammatory effects of an AT extract, and demonstrates its potential in the treatment of inflammatory diseases.

PMID:
24534870
DOI:
10.3892/mmr.2014.1941
[Indexed for MEDLINE]
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