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Am J Trop Med Hyg. 2014 Apr;90(4):612-6. doi: 10.4269/ajtmh.13-0708. Epub 2014 Feb 17.

Infection of laboratory-colonized Anopheles darlingi mosquitoes by Plasmodium vivax.

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Division of Infectious Diseases, Department of Medicine, University of California at San Diego, La Jolla, California; Laboratorio ICEMR-Amazonia, Laboratorios de Investigacion y Desarrollo, Facultad de Ciencias y Filosofia, Universidad Peruana Cayetano Heredia, Lima, Perú; Instituto de Medicine Tropical "Alexander von Humboldt," Universidad Peruana Cayetano Heredia, Lima, Perú; Directorate of Health, Iquitos, Peru; Department of Pediatrics, University of California at San Diego School of Medicine, La Jolla, California; Wadsworth Center, New York State Department of Health, Albany, New York; Department of Biomedical Sciences, School of Public Health, State University of New York, Albany, New York.


Anopheles darlingi Root is the most important malaria vector in the Amazonia region of South America. However, continuous propagation of An. darlingi in the laboratory has been elusive, limiting entomological, genetic/genomic, and vector-pathogen interaction studies of this mosquito species. Here, we report the establishment of an An. darlingi colony derived from wild-caught mosquitoes obtained in the northeastern Peruvian Amazon region of Iquitos in the Loreto Department. We show that the numbers of eggs, larvae, pupae, and adults continue to rise at least to the F6 generation. Comparison of feeding Plasmodium vivax ex vivo of F4 and F5 to F1 generation mosquitoes showed the comparable presence of oocysts and sporozoites, with numbers that corresponded to blood-stage asexual parasitemia and gametocytemia, confirming P. vivax vectorial capacity in the colonized mosquitoes. These results provide new avenues for research on An. darlingi biology and study of An. darlingi-Plasmodium interactions.

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