Format

Send to

Choose Destination
Acta Biomater. 2014 Jun;10(6):2855-65. doi: 10.1016/j.actbio.2014.01.034. Epub 2014 Feb 15.

Increased bone remodelling around titanium implants coated with chondroitin sulfate in ovariectomized rats.

Author information

1
Department of Trauma and Reconstructive Surgery, University Hospital "Carl Gustav Carus", Technische Universität Dresden, Fetscherstrasse 74, Dresden 01307, Germany; Institute of Immunology, Medical Faculty "Carl Gustav Carus", Technische Universität Dresden, Fetscherstrasse 74, Dresden 01307, Germany. Electronic address: Jan.Dudeck@tu-dresden.de.
2
Department of Trauma and Reconstructive Surgery, University Hospital "Carl Gustav Carus", Technische Universität Dresden, Fetscherstrasse 74, Dresden 01307, Germany.
3
Max Bergmann Center of Biomaterials, Technische Universität Dresden, Budapester Str. 27, Dresden 01069, Germany.
4
Institute for Zoology, Chair for Molecular Cell Physiology and Endocrinology, Technische Universität Dresden, Zellescher Weg 2b, Dresden 01062, Germany.
5
Biomedical Engineering Division, School of Bio-Sciences and Technology, VIT University, Vellore, Tamilnadu 623014, India; Max Planck Institute of Colloids and Interfaces, Department of Biomaterials, Potsdam-Golm Science Park, Am Mühlenberg 1, Potsdam 14476, Germany.
6
BAM Federal Institute for Materials Research and Testing, Unter den Eichen 87, Berlin 12205, Germany.
7
Max Planck Institute of Colloids and Interfaces, Department of Biomaterials, Potsdam-Golm Science Park, Am Mühlenberg 1, Potsdam 14476, Germany.
8
Department of Trauma and Reconstructive Surgery, University Hospital "Carl Gustav Carus", Technische Universität Dresden, Fetscherstrasse 74, Dresden 01307, Germany; Center for Regenerative Therapies Dresden (CRTD), Tatzberg 47, Dresden 01307, Germany.

Abstract

Coating titanium implants with artificial extracellular matrices based on collagen and chondroitin sulfate (CS) has been shown to enhance bone remodelling and de novo bone formation in vivo. The aim of this study was to evaluate the effect of estrogen deficiency and hormone replacement therapy (HRT) on the osseointegration of CS-modified Ti implants. 30 adult female, ovariectomized Wistar rats were fed either with an ethinyl-estradiol-rich diet (E) to simulate a clinical relevant HRT or with a genistein-rich diet (G) to test an alternative therapy based on nutritionally relevant phytoestrogens. Controls (C) received an estrogen-free diet. Uncoated titanium pins (Ti) or pins coated with type-I collagen and CS (Ti/CS) were inserted 8weeks after ovarectomy into the tibia. Specimens were retrieved 28days after implantation. Both the amount of newly formed bone and the affinity index (P<0.05) were moderately higher around Ti/CS implants as compared to uncoated Ti. The highest values were measured in the G-Ti/CS and E-Ti/CS groups, the lowest values for the E-Ti and G-Ti controls. Quantitative synchrotron radiation micro-computed tomography (SRμCT) revealed the highest increase in total bone formation around G-Ti/CS as compared to C-Ti (P<0.01). The effects with respect to direct bone apposition were less pronounced with SRμCT. Using scanning nanoindentation, both the indentation modulus and the hardness of the newly formed bone were highest in the E-Ti/CS, G-Ti/CS and G-Ti groups as compared to C-Ti (P<0.05). Coatings with collagen and CS appear to improve both the quantity and quality of bone formed around Ti implants in ovarectomized rats. A simultaneous ethinyl estradiol- and genistein-rich diet seems to enhance these effects.

KEYWORDS:

Chondroitin sulfate; Estrogen replacement therapy; Osseointegration; Osteoporosis; Scanning nanoindentation

PMID:
24534718
DOI:
10.1016/j.actbio.2014.01.034
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center