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Clin Chem. 1988 May;34(5):975-7.

Interference of fetal hemoglobin with the spectrophotometric measurement of carboxyhemoglobin.

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1
Department of Pediatrics, Stanford University School of Medicine, CA 94305-5119.

Abstract

We measured the concentration of carboxyhemoglobin (HbCO) in blood samples from 32 neonates by spectrophotometry (IL282 CO-Oximeter) and gas chromatography, finding a strong positive correlation (r = 0.89) between the concentration of fetal hemoglobin (Hb F) and HbCO as measured by spectrophotometry, but not by gas chromatography. Thus, Hb F interferes with the determination of HbCO by spectrophotometric techniques by falsely increasing apparent HbCO in direct proportion to Hb F. We conclude that, when Hb F is known or suspected to be present, blood HbCO cannot be reliably determined by methods based on spectrophotometry.

PMID:
2453310
[Indexed for MEDLINE]
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