Format

Send to

Choose Destination
Mol Biol Evol. 2014 May;31(5):1292-4. doi: 10.1093/molbev/msu074. Epub 2014 Feb 13.

Ancient whole genome enrichment using baits built from modern DNA.

Author information

1
McMaster Ancient DNA Centre, Department of Anthropology, McMaster University, Hamilton, ON, Canada.

Abstract

We report metrics from complete genome capture of nuclear DNA from extinct mammoths using biotinylated RNAs transcribed from an Asian elephant DNA extract. Enrichment of the nuclear genome ranged from 1.06- to 18.65-fold, to an apparent maximum threshold of ∼80% on-target. This projects an order of magnitude less costly complete genome sequencing from long-dead organisms, even when a reference genome is unavailable for bait design.

KEYWORDS:

ancient DNA; targeted enrichment; whole-genome capture

PMID:
24531081
DOI:
10.1093/molbev/msu074
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Silverchair Information Systems
Loading ...
Support Center