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FEBS Lett. 2014 Mar 18;588(6):1008-15. doi: 10.1016/j.febslet.2014.01.062. Epub 2014 Feb 13.

Cyclic mononucleotides modulate potassium and calcium flux responses to H2O2 in Arabidopsis roots.

Author information

1
Biological and Environmental Sciences & Engineering Division, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia.
2
Bioscience Core Facility, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia.
3
Department of Applied Biology, University of Perugia, Borgo XX Giugno, 74, 06121 Perugia, Italy.
4
School of Agricultural Science, University of Tasmania, Private Bag 54, Hobart, Tasmania 7001, Australia.
5
Biological and Environmental Sciences & Engineering Division, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia. Electronic address: christoph.gehring@KAUST.edu.sa.

Abstract

Cyclic mononucleotides are messengers in plant stress responses. Here we show that hydrogen peroxide (H2O2) induces rapid net K(+)-efflux and Ca(2+)-influx in Arabidopsis roots. Pre-treatment with either 10μM cAMP or cGMP for 1 or 24h does significantly reduce net K(+)-leakage and Ca(2+)-influx, and in the case of the K(+)-fluxes, the cell permeant cyclic mononucleotides are more effective. We also examined the effect of 10μM of the cell permeant 8-Br-cGMP on the Arabidopsis microsomal proteome and noted a specific increase in proteins with a role in stress responses and ion transport, suggesting that cGMP is sufficient to directly and/or indirectly induce complex adaptive changes to cellular stresses induced by H2O2.

KEYWORDS:

Arabidopsis thaliana; Cyclic mononucleotide; Hydrogen peroxide; Ion flux; Plant stress; Proteomic; cAMP; cGMP

PMID:
24530500
DOI:
10.1016/j.febslet.2014.01.062
[Indexed for MEDLINE]
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