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Cell. 2014 Feb 13;156(4):691-704. doi: 10.1016/j.cell.2014.01.039.

The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants.

Author information

1
Department of Plant Systems Biology, VIB, Technologiepark 927, 9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
2
Max-Planck-Institut für Moleculare Planzenphysiologie, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany.
3
Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA.
4
Department of Plant Systems Biology, VIB, Technologiepark 927, 9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium; Institute of Science and Technology (IST Austria), Am Campus 1, 3400 Klosterneuburg, Austria.
5
Max-Planck-Institut für Moleculare Planzenphysiologie, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany; Institute of Biotechnology (IBT), University of Vilnius, V. Graiciuno 8, 02242 Vilnius, Lithuania.
6
Laboratory of Cell Biology, Wageningen University, Droevendaalsesteeg 1, 6708PB Wageningen, The Netherlands.
7
Department of Medical Protein Research, VIB, A. Baertsoenkaai 3, 9000 Ghent, Belgium; Department of Biochemistry, Ghent University, 9000 Ghent, Belgium.
8
Department of Biology, Center for Proteome Analysis and Mass Spectrometry, University of Antwerp, 2020 Antwerp, Belgium; Flemish Institute for Technological Research (VITO), 2400 Mol, Belgium.
9
Max-Planck-Institut für Moleculare Planzenphysiologie, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany. Electronic address: persson@mpimp-golm.mpg.de.
10
Department of Plant Systems Biology, VIB, Technologiepark 927, 9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium. Electronic address: daniel.vandamme@psb.vib-ugent.be.

Abstract

Clathrin-mediated endocytosis is the major mechanism for eukaryotic plasma membrane-based proteome turn-over. In plants, clathrin-mediated endocytosis is essential for physiology and development, but the identification and organization of the machinery operating this process remains largely obscure. Here, we identified an eight-core-component protein complex, the TPLATE complex, essential for plant growth via its role as major adaptor module for clathrin-mediated endocytosis. This complex consists of evolutionarily unique proteins that associate closely with core endocytic elements. The TPLATE complex is recruited as dynamic foci at the plasma membrane preceding recruitment of adaptor protein complex 2, clathrin, and dynamin-related proteins. Reduced function of different complex components severely impaired internalization of assorted endocytic cargoes, demonstrating its pivotal role in clathrin-mediated endocytosis. Taken together, the TPLATE complex is an early endocytic module representing a unique evolutionary plant adaptation of the canonical eukaryotic pathway for clathrin-mediated endocytosis.

PMID:
24529374
DOI:
10.1016/j.cell.2014.01.039
[Indexed for MEDLINE]
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