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Carbohydr Polym. 2014 Mar 15;103:140-2. doi: 10.1016/j.carbpol.2013.12.029. Epub 2013 Dec 18.

Cellulase-assisted extraction and antibacterial activity of polysaccharides from the dandelion Taraxacum officinale.

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1
School of Marine Science and Technology, Huaihai Institute of Technology, 59 Changwu Road, Lianyungang 222005, China. Electronic address: whbhhit@sina.com.

Abstract

In the present study, we investigated the cellulase-assisted extraction and antibacterial activity of water-soluble polysaccharides from the dandelion Taraxacum officinale. The extraction conditions, optimized for improving yield, were as follows: time, 46.11 min; temperature, 54.87 °C; pH, 4.51 and cellulase enzyme, 4000 U/g. Under these conditions, the yield of polysaccharides from dandelion (PD) reached 20.67% (w/w). The sugar content of PD was 95.6% (w/w), and it displayed high antibacterial activity at a concentration of 100mg/mL against Escherichia coli, Bacillus subtilis and Staphylococcus aureus. These results indicate that PD may be a viable option for use as a food preservative.

KEYWORDS:

Antibacterial activities; Cellulase-assisted extraction; Polysaccharides

PMID:
24528711
DOI:
10.1016/j.carbpol.2013.12.029
[Indexed for MEDLINE]
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