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Nutr Hosp. 2014 Feb 1;29(2):388-92. doi: 10.3305/nh.2014.29.2.7080.

Anti-proliferative action of silibinin on human colon adenomatous cancer HT-29 cells.

Author information

1
Department of Pharmacology Postgraduate Institute of Medical Education and Research. Chandigarh.. sanyalpu@gmail.com.
2
Faculty of Pharmacy. Jamia Hamdard. New Delhi.. sanyalpu@gmail.com.
3
Department of Experimental Medicine and Biotechnology. Postgraduate Institute of Medical Education and Research. Chandigarh.. sanyalpu@gmail.com.
4
Department of Biophysics. Panjab University. Chandigarh.. sanyalpu@gmail.com.

Abstract

in English, Spanish

BACKGROUND:

Silibinin a flavonoid from milk thistle (Silybum marianum) exhibit a variety of pharmacological actions, including anti-proliferative and apoptotic activities against various types of cancers in intact animals and cancer cell lines. In the present study, the effect of silibinin on human colon cancer HT-29 cells was studied.

METHOD:

Incubations of cells with different silibinin concentrations (0.783-1,600 ug/ml) for 24, 48 or 72 h showed a progressive decline in cell viability.

RESULTS:

Loss of cell viability was time dependent and optimum inhibition of cell growth (78%) was observed at 72 h. Under inverted microscope, the dead cells were seen as cell aggregates. IC50 (silibinin concentration killing 50% cells) values were 180, 110 and 40 ug/ml at 24, 48 and 72 h respectively.

CONCLUSION:

These findings re-enforce the anticancer potential of silibinin, as reported earlier for various other cancer cell lines (Ramasamy and Agarwal (2008), Cancer Letters, 269: 352-62).

PMID:
24528358
DOI:
10.3305/nh.2014.29.2.7080
[Indexed for MEDLINE]
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