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Stem Cell Reports. 2014 Jan 23;2(2):205-18. doi: 10.1016/j.stemcr.2013.12.007. eCollection 2014 Feb 11.

Characterization of human induced pluripotent stem cell-derived retinal pigment epithelium cell sheets aiming for clinical application.

Author information

1
Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, Kobe, Hyogo 650-0047, Japan ; Department of Ophthalmology, Kawasaki Medical School, Kurashiki, Okayama 701-0114, Japan.
2
Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, Kobe, Hyogo 650-0047, Japan.
3
Department of Ophthalmology, Kawasaki Medical School, Kurashiki, Okayama 701-0114, Japan.

Abstract

Age-related macular degeneration (AMD) causes severe visual impairment due in part to age-dependent impairment of retinal pigment epithelium (RPE). It has been suggested that autologous human induced pluripotent stem cells (hiPSCs) may represent a useful cell source for the generation of graft RPE. We generated hiPSC-derived RPE (hiPSC-RPE) cell sheets optimized to meet clinical use requirements, including quality, quantity, consistency, and safety. These cell sheets are generated as a monolayer of cells without any artificial scaffolds, express typical RPE markers, form tight junctions that exhibit polarized secretion of growth factors, and show phagocytotic ability and gene-expression patterns similar to those of native RPE. Additionally, upon transplantation, autologous nonhuman primate iPSC-RPE cell sheets showed no immune rejection or tumor formation. These results suggest that autologous hiPSC-RPE cell sheets may serve as a useful form of graft for use in tissue replacement therapy for AMD.

PMID:
24527394
PMCID:
PMC3923225
DOI:
10.1016/j.stemcr.2013.12.007
[Indexed for MEDLINE]
Free PMC Article

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