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Stem Cell Reports. 2014 Feb 6;2(2):171-9. doi: 10.1016/j.stemcr.2014.01.002. eCollection 2014 Feb 11.

Identification of specific cell-surface markers of adipose-derived stem cells from subcutaneous and visceral fat depots.

Author information

1
Fat Metabolism and Stem Cell Group, Laboratory of Metabolic Medicine, Singapore Bioimaging Consortium, A STAR, Helios, 11 Biopolis Way, Singapore 138667, Singapore.
2
Saw Swee Hock School of Public Health, National University of Singapore, MD3, 16 Medical Drive, Singapore 117597, Singapore.
3
Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, MD6, 14 Medical Drive, Singapore 117599, Singapore.
4
Department of Surgery, National University Hospital, 5 Lower Kent Ridge Road, Singapore 119074, Singapore.
5
Cardiovascular and Metabolic Disorders Program, Duke-NUS Graduate Medical School, 8 College Road, Singapore 169857, Singapore ; Laboratory of Metabolic Medicine, Singapore Bioimaging Consortium and Metabolism in Human Diseases Unit, Institute of Molecular and Cell Biology, A STAR, Singapore 138667, Singapore.
6
Fat Metabolism and Stem Cell Group, Laboratory of Metabolic Medicine, Singapore Bioimaging Consortium, A STAR, Helios, 11 Biopolis Way, Singapore 138667, Singapore ; Cardiovascular and Metabolic Disorders Program, Duke-NUS Graduate Medical School, 8 College Road, Singapore 169857, Singapore.

Abstract

Adipose-derived stem/stromal cells (ASCs) from the anatomically distinct subcutaneous and visceral depots of white adipose tissue (WAT) differ in their inherent properties. However, little is known about the molecular identity and definitive markers of ASCs from these depots. In this study, ASCs from subcutaneous fat (SC-ASCs) and visceral fat (VS-ASCs) of omental region were isolated and studied. High-content image screening of over 240 cell-surface markers identified several potential depot-specific markers of ASCs. Subsequent studies revealed consistent predominant expression of CD10 in SC-ASCs and CD200 in VS-ASCs across 12 human subjects and in mice. CD10-high-expressing cells sorted from SC-ASCs differentiated better than their CD10-low-expressing counterparts, whereas CD200-low VS-ASCs differentiated better than CD200-high VS-ASCs. The expression of CD10 and CD200 is thus depot-dependent and associates with adipogenic capacities. These markers will offer a valuable tool for tracking and screening of depot-specific stem cell populations.

PMID:
24527391
PMCID:
PMC3923222
DOI:
10.1016/j.stemcr.2014.01.002
[Indexed for MEDLINE]
Free PMC Article

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