Format

Send to

Choose Destination
See comment in PubMed Commons below
Nat Commun. 2014;5:3309. doi: 10.1038/ncomms4309.

Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography.

Author information

1
Department of Physics, Arizona State University, Tempe, Arizona 85287, USA.
2
The Scripps Research Institute, Department of Integrative Structural and Computational Biology, La Jolla, California 92037, USA.
3
Center for Free-Electron Laser Science, DESY, Notkestrasse 85, Hamburg 22607, Germany.
4
Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287, USA.
5
SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
6
1] SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA [2] Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-751 24, Sweden.
7
1] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, Hamburg 22607, Germany [2] Departamento de Química, Universidad Autónoma de Madrid, Ciudad Universitaria de Cantoblanco, Madrid 28049, Spain.
8
Max-Planck-Institut für medizinische Forschung, Jahnstrasse 29, Heidelberg 69120, Germany.
9
1] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, Hamburg 22607, Germany [2] Department of Physics, University of Hamburg, Hamburg 22761, Germany [3] Center for Ultrafast Imaging, Hamburg 22607, Germany.
10
1] Department of Physics, Arizona State University, Tempe, Arizona 85287, USA [2] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, Hamburg 22607, Germany.
11
School of Medicine and School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland.

Abstract

Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-μm-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.

PMID:
24525480
PMCID:
PMC4061911
DOI:
10.1038/ncomms4309
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group Icon for PubMed Central
    Loading ...
    Support Center