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Food Chem Toxicol. 2014 May;67:72-9. doi: 10.1016/j.fct.2014.02.004. Epub 2014 Feb 10.

High concentrations of genistein exhibit pro-oxidant effects in primary muscle cells through mechanisms involving 5-lipoxygenase-mediated production of reactive oxygen species.

Author information

1
Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, PR China; The Key Laboratory of Animal Nutrition and Feed Science (South China) of Ministry of Agriculture, Guangzhou, PR China; State Key Laboratory of Livestock and Poultry Breeding, Guangzhou, PR China; Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangzhou, PR China; Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou, PR China. Electronic address: cwei010230@163.com.
2
Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, PR China; The Key Laboratory of Animal Nutrition and Feed Science (South China) of Ministry of Agriculture, Guangzhou, PR China; State Key Laboratory of Livestock and Poultry Breeding, Guangzhou, PR China; Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangzhou, PR China; Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou, PR China. Electronic address: lyc0123@tom.com.
3
Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, PR China; The Key Laboratory of Animal Nutrition and Feed Science (South China) of Ministry of Agriculture, Guangzhou, PR China; State Key Laboratory of Livestock and Poultry Breeding, Guangzhou, PR China; Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangzhou, PR China; Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou, PR China. Electronic address: xianyongm@gmail.com.
4
Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, PR China; The Key Laboratory of Animal Nutrition and Feed Science (South China) of Ministry of Agriculture, Guangzhou, PR China; State Key Laboratory of Livestock and Poultry Breeding, Guangzhou, PR China; Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangzhou, PR China; Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou, PR China. Electronic address: jiangz41@163.com.
5
Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, PR China; The Key Laboratory of Animal Nutrition and Feed Science (South China) of Ministry of Agriculture, Guangzhou, PR China; State Key Laboratory of Livestock and Poultry Breeding, Guangzhou, PR China; Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangzhou, PR China; Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou, PR China. Electronic address: 619836899@qq.com.

Abstract

Genistein, a typical soy isoflavone, is an important antioxidant for improving human health and animal production but the compound possesses some pro-oxidant potential. In order to explore the latter, the dose-response relationship of various concentrations of genistein on both cellular proliferation and the redox system were examined. The proliferation of primary muscle cells was promoted by a low concentration of genistein but was inhibited by high concentrations, which also enhanced lipid oxidation and suppressed membrane fluidity. By selecting a high concentration (200 μM) as a pro-oxidant treatment, the mechanism underlying the pro-oxidant function of genistein was then explored. The generation of intracellular reactive oxygen species (ROS) was stimulated by 200 μM genistein, with inhibited expression of NADPH oxidase 4 and cyclooxygenase 1 and 2 as well as increased activity of the glutathione redox system. The cellular expression of 5-lipoxygenase, however, was up-regulated by 200 μM genistein and the addition of 5-lipoxygenase inhibitor (Zileuton) decreased genistein-induced intracellular ROS level, close to that from the addition of the ROS scavenger, N-acetylcysteine. It is concluded that higher concentrations of genistein exert pro-oxidant potential in the primary muscle cells through enhancing ROS production in a 5-lipoxygenase-dependent manner.

KEYWORDS:

5-Lipoxygenase; Genistein; Primary muscle cells; Pro-oxidant; Reactive oxygen species

PMID:
24525097
DOI:
10.1016/j.fct.2014.02.004
[Indexed for MEDLINE]
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