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Nanomedicine. 2014 Jul;10(5):949-58. doi: 10.1016/j.nano.2014.01.010. Epub 2014 Feb 10.

Stem cell responses to plasma surface modified electrospun polyurethane scaffolds.

Author information

1
Bionano Systems Laboratory, Department of Microtechnology and Nanoscience (MC2), Chalmers University of Technology, Göteborg, Sweden.
2
Center for Brain Repair and Rehabilitation (CBR) Institute for Neuroscience and Physiology, Gothenburg, Sweden.
3
Bionano Systems Laboratory, Department of Microtechnology and Nanoscience (MC2), Chalmers University of Technology, Göteborg, Sweden; Key Laboratory of Advanced Display and System Applications & SMIT Center, Shanghai University, Mechatronical Engineering and Automation Building, Shanghai, People's Republic of China. Electronic address: johan.liu@chalmers.se.

Abstract

The topographical effects from functional materials on stem cell behavior are currently of interest in tissue engineering and regenerative medicine. Here we investigate the influence of argon, oxygen, and hydrogen plasma surface modification of electrospun polyurethane fibers on human embryonic stem cell (hESC) and rat postnatal neural stem cell (NSC) responses. The plasma gases were found to induce three combinations of fiber surface functionalities and roughness textures. On randomly oriented fibers, plasma treatments lead to substantially increased hESC attachment and proliferation as compared to native fibers. Argon plasma was found to induce the most optimal combination of surface functionality and roughness for cell expansion. Contact guided migration of cells and alignment of cell processes were observed on aligned fibers. Neuronal differentiation around 5% was found for all samples and was not significantly affected by the induced variations of surface functional group distribution or individual fiber topography.

FROM THE CLINICAL EDITOR:

In this study the influence of argon, oxygen, and hydrogen plasma surface modification of electrospun polyurethane fibers on human embryonic stem cell and rat postnatal neural stem cell (NSC) responses is studied with the goal of clarifying the potential effects of functional materials on stem cell behavior, a topic of substantial interest in tissue engineering and regenerative medicine.

KEYWORDS:

Polyurethane; Scaffold; Stem cell; Surface modification

PMID:
24524929
DOI:
10.1016/j.nano.2014.01.010
[Indexed for MEDLINE]

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