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J Vet Intern Med. 2014 Mar-Apr;28(2):515-21. doi: 10.1111/jvim.12317. Epub 2014 Feb 13.

Breed distribution of SOD1 alleles previously associated with canine degenerative myelopathy.

Author information

1
Department of Veterinary Pathology, University of Missouri, Columbia, MO.

Abstract

BACKGROUND:

Previous reports associated 2 mutant SOD1 alleles (SOD1:c.118A and SOD1:c.52T) with degenerative myelopathy in 6 canine breeds. The distribution of these alleles in other breeds has not been reported.

OBJECTIVE:

To describe the distribution of SOD1:c.118A and SOD1:c.52T in 222 breeds.

ANIMALS:

DNA from 33,747 dogs was genotyped at SOD1:c.118, SOD1:c.52, or both. Spinal cord sections from 249 of these dogs were examined.

METHODS:

Retrospective analysis of 35,359 previously determined genotypes at SOD1:c.118G>A or SOD1:c.52A>T and prospective survey to update the clinical status of a subset of dogs from which samples were obtained with a relatively low ascertainment bias.

RESULTS:

The SOD1:c.118A allele was found in cross-bred dogs and in 124 different canine breeds whereas the SOD1:c.52T allele was only found in Bernese Mountain Dogs. Most of the dogs with histopathologically confirmed degenerative myelopathy were SOD1:c.118A homozygotes, but 8 dogs with histopathologically confirmed degenerative myelopathy were SOD1:c.118A/G heterozygotes and had no other sequence variants in their SOD1 amino acid coding regions. The updated clinical conditions of dogs from which samples were obtained with a relatively low ascertainment bias suggest that SOD1:c.118A homozygotes are at a much higher risk of developing degenerative myelopathy than are SOD1:c.118A/G heterozygotes.

CONCLUSIONS AND CLINICAL IMPORTANCE:

We conclude that the SOD1:c.118A allele is widespread and common among privately owned dogs whereas the SOD1:c.52T allele is rare and appears to be limited to Bernese Mountain Dogs. We also conclude that breeding to avoid the production of SOD1:c.118A homozygotes is a rational strategy.

KEYWORDS:

Amyotrophic lateral sclerosis; Cytoplasmic aggregates; DNA test; Spinal cord

PMID:
24524809
PMCID:
PMC4238831
DOI:
10.1111/jvim.12317
[Indexed for MEDLINE]
Free PMC Article

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