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Chem Biol Drug Des. 2014 Jul;84(1):108-15. doi: 10.1111/cbdd.12300. Epub 2014 May 12.

In vitro evaluation of doxorubicin-incorporated magnetic albumin nanospheres.

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1
Department of Chemistry, Faculty of Science, İzmir Institute of Technology, 35430, Urla, İzmir, Turkey.

Abstract

Magnetic albumin nanospheres that incorporate doxorubicin (M-DOX-BSA-NPs) were prepared previously by our research group to develop magnetically responsive drug carrier system. This nanocarrier was synthesized as a drug delivery system for targeted chemotherapy. In this work, cytotoxic effects of doxorubicin (DOX)-loaded/unloaded or magnetic/non-magnetic nanoparticles and free DOX against PC-3 cells and A549 cells were determined with the MTT test and the results were compared with each other. DOX-loaded magnetic albumin nanospheres (M-DOX-BSA-NPs) were found more cytotoxic than other formulations. The quantitative data obtained from flow cytometry analysis further verified the higher targeting and killing ability of M-DOX-BSA-NPs than free DOX on both of the cancer cell lines. Additionally, the results of cell cycle analysis have showed that M-DOX-BSA-NPs affected G1 and G2 phases. Finally, cell images were obtained using spin-disk confocal microscopy, and cellular uptake of M-DOX-BSA-NPs was visualized. The findings of this study suggest that M-DOX-BSA-NPs represent a potential doxorubicin delivery system for targeted drug transport into prostate and lung cancer cells.

KEYWORDS:

A549 cell line; PC3 cell line; apoptosis; doxorubicin; magnetic albumin nanoparticle; targeted drug delivery

PMID:
24524300
DOI:
10.1111/cbdd.12300
[Indexed for MEDLINE]
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