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Avicenna J Med Biotechnol. 2014 Jan;6(1):47-52.

Fourier transform infrared spectroscopy: a potential technique for noninvasive detection of spermatogenesis.

Author information

1
Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
2
Department of Chemistry, Sharif University of Technology, Tehran, Iran.

Abstract

BACKGROUND:

The seminal plasma is an excellent source for noninvasive detection of spermatogenesis. The seminal plasma of normospermic and azoospermic men has been analyzed for detection of spermatogenesis.

METHODS:

Optical spectroscopy (Attenuated Total Reflectance-Infrared spectroscopy (ATR-IR) and Fourier Transform infrared spectroscopy (FT-IR) has been used to analyze the seminal plasma and the metabolome of seminal plasma for detection of spermatogenesis.

RESULTS:

The seminal plasma of normospermic and azoospermic men has been analyzed by ATR-IR. The results show that there is a pattern variation in the azoospermic men compared to normospermic men. However, the seminal plasma is too complex to show significant pattern variation. Therefore, the metabolome which is a subcomponent of the seminal plasma was analyzed. The seminal plasma metabolome of normospermic and azoospermic men has been analyzed by FT-IR. A significant pattern change was observed. The data combined with chemometrics analysis showed that significant changes are observed at metabolome level.

CONCLUSION:

We suggest that FT-IR has the potential as a diagnostic tool instead of testicular biopsy.

KEYWORDS:

Azoospermia; Fourier transform infrared spectroscopy; Metabolome; Seminal plasma

PMID:
24523955
PMCID:
PMC3895579

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